 Research Article
 Open Access
Assessing the Exceptionality of Coloured Motifs in Networks
 Sophie Schbath^{1}Email author,
 Vincent Lacroix^{2} and
 MarieFrance Sagot^{3, 4, 5}
https://doi.org/10.1155/2009/616234
© The Author(s) 2009
 Received: 1 June 2008
 Accepted: 11 October 2008
 Published: 26 October 2008
Abstract
Various methods have been recently employed to characterise the structure of biological networks. In particular, the concept of network motif and the related one of coloured motif have proven useful to model the notion of a functional/evolutionary building block. However, algorithms that enumerate all the motifs of a network may produce a very large output, and methods to decide which motifs should be selected for downstream analysis are needed. A widely used method is to assess if the motif is exceptional, that is, over or underrepresented with respect to a null hypothesis. Much effort has been put in the last thirty years to derive values for the frequencies of topological motifs, that is, fixed subgraphs. They rely either on (compound) Poisson and Gaussian approximations for the motif count distribution in ErdösRényi random graphs or on simulations in other models. We focus on a different definition of graph motifs that corresponds to coloured motifs. A coloured motif is a connected subgraph with fixed vertex colours but unspecified topology. Our work is the first analytical attempt to assess the exceptionality of coloured motifs in networks without any simulation. We first establish analytical formulae for the mean and the variance of the count of a coloured motif in an ErdösRényi random graph model. Using simulations under this model, we further show that a PólyaAeppli distribution better approximates the distribution of the motif count compared to Gaussian or Poisson distributions. The PólyaAeppli distribution, and more generally the compound Poisson distributions, are indeed well designed to model counts of clumping events. Altogether, these results enable to derive a value for a coloured motif, without spending time on simulations.
Keywords
 Metabolic Network
 Random Graph
 Biological Network
 Network Motif
 Random Graph Model
1. Introduction
Descriptions of biological networks serve two main purposes. On the one hand, it enables to address questions related to the evolution of the network, that is, how such a complex structure has been set up in the course of evolution. On the other hand, structural analysis can be seen as a first necessary step prior to a dynamical analysis which in turn enables to simulate networks and to study their response to perturbation. Usually, three main classes of biological networks are considered [1]: protein interaction, gene regulatory, and metabolic. When analysing their structure, these networks are usually modelled as graphs, where vertices represent molecules (metabolites, genes, and proteins) and edges (directed or undirected) represent interactions between these molecules (the direction, when it is known, indicating which molecule is acting upon the other). For instance, in the case of a gene regulatory network, vertices correspond to genes and there is a directed edge from a gene coding for a transcription factor to every gene that this transcription factor regulates.
The structure of a biological network may be apprehended by using a variety of measures, such as vertex degree [2], degree correlation [3], or average shortest path length [4].
In this paper, we focus on the concept of motif. A network motif has been initially defined as a pattern of interconnections which occurs unexpectedly often in a network [5, 6]. The assumption generally made is that subnetworks sharing the same topology will be functionally similar. Over (resp., under) represented subnetworks may therefore correspond to conserved (resp., avoided) and thus important (resp., vital/detrimental) cellular functions. In the context of regulatory networks, simple patterns such as loops may be interpreted as logical circuits controlling the dynamic behaviour of a network. If the over and underrepresentations of network motifs are often assessed via simulations of random networks in practice, approximations of the subgraph count distribution in various random graph models have been proposed in the literature. Some of these approximations can be found in the book by Janson et al. [7] or in more recent studies such as those by Stark [8], Itzkovitz et al. [9], Camacho et al. [10], and Picard et al. [11].
Moreover, in some situations, as, for example, in the case of protein interaction networks, the topology of the network is not fully known. Indeed, highthroughput experiments used to obtain largescale protein interaction data are notoriously noisy, that is, they may detect interactions when there is none (false positive) and they may miss existing interactions (false negative). In this context, it may be inadequate to look for exact repetitions of a pattern. An alternative definition has thus been proposed, where a motif is defined by using the labels of its vertices and only connectedness of the induced subgraph is required [12].
A coloured motif is defined as a multiset of colours (vertex labels), that is, a motif may contain colours whose multiplicity are greater than 1. The cardinality of a motif, that is, of the multiset, will be called the size of a motif. An occurrence of a motif is defined as a connected subgraph whose labels match the motif.
The enumeration of coloured motifs is a nontrivial task which has been the subject of several works [12, 13] which allowed to establish the complexity of the problem and provide algorithms to efficiently detect all the occurrences of a motif in a graph. In practice, current methods now allow to enumerate all the motifs of size 7 of a graph representing the metabolic network of a bacterium in less than two hours. Beyond the time complexity of the task, a major challenge that remains open is to make sense of the potentially very large output of such an enumeration procedure, especially when the focus is not on a single motif but on all motifs of a given size. Ideally, one would need a method to rank the motifs according to their biological relevance in order to prioritise a small number of motifs for downstream analysis. However, the notion of biological relevance is generally ill defined, and a classically used approximation is its statistical significance (or exceptionality).
The exceptionality of a coloured motif, that is the over or underrepresentation of the motif with respect to a null model, can be assessed by comparing the observed count of occurrences of a motif to the expected count of the same motif under a null hypothesis. Up to now, this procedure was performed (e.g., in MOTUS [14], http://pbil.univlyon1.fr/software/motus/) using simulations: a large number of random graphs were generated and the motif of interest was sought in each one, generating an empirical distribution of the motif count to which the observed count could be compared in order to derive a score and a value. The main limitation of this procedure is that it adds a multiplicative factor to the time complexity of the algorithm. Moreover, it is not trivial to choose the optimal number of simulations to perform in order to get a satisfactory estimation of the value. As a rule of thumb, in order to estimate quite accurately a value of 1 over , at least simulations should be performed.
In this paper, we propose a new approach for assessing the exceptionality of coloured motifs which do not require simulations and therefore circumvents the previously mentioned limitations. We were able to establish exact analytical formulae for the mean and the variance of the count of a coloured motif in an ErdösRényi (ER) random graph model. Thanks to these results, one can now derive a score for each motif and therefore rank them according to their exceptionality. We then worked on modelling the complete distribution of the count of a coloured motif in an ER random graph model. To this purpose, we performed a large number of simulations, using different colour frequencies for the motif and different number of vertices and edges for the graph. We could establish that the Poisson distribution was not appropriate whereas the PólyaAeppli distribution was a good and better approximation than the commonly used Gaussian distribution. The choice of a PólyaAeppli distribution was driven by the following facts: (i) motif occurrences overlap in a network, as shown in Figure 1; (ii) compound Poisson distributions are particularly adapted to model counts of clumping events [15, Chapter 9]; (iii) PólyaAeppli approximations are efficient for the count of words in letter sequences [16]. These results can in turn be used to derive a value for each motif, and, therefore, to introduce a cutoff for deciding which motifs should be selected for downstream analysis.
To our knowledge, there has been no previous work on the significance of coloured motifs in random graphs. This is the reason why we started by focusing on the more general random graph model that is available. We are aware that this may not be the most suitable model to describe the structure of a biological network. However, we argue that this work provides a first necessary basis which can later be extended to richer models, such as the promising mixture of ErdösRényi models proposed by Daudin et al. [17].
2. Definitions and Notations
Coloured Random Graph Model
We consider a random graph with vertices . We assume that random edges are independent and distributed according to a Bernoulli distribution with parameter (the socalled ErdösRényi model). Moreover, vertices are randomly and independently coloured as follows. Let be a finite set of different colours and a probability measure on : is then the probability for a vertex to be coloured with .
In a metabolic network, the colours of reaction vertices can represent classes of chemical transformations; in regulation networks, the colours of gene vertices can represent functional classes. For defining these classes, the EC number hierarchy (http://www.chem.qmul.ac.uk/iubmb/enzyme/) or Gene Ontology (http://www.geneontology.org/GO.doc.shtml) is classically used.
Coloured Motif
We consider motifs as introduced in Lacroix et al. [12]: a (coloured) motif of size is a multiset of colours . Colours from a motif may not be different, that is, one may have for some . We then denote by the multiplicity of the colour in . When there is no ambiguity, will simply be denoted by . The notion of multiplicity of a single colour in will be extended to a multiset of colours in Section 3.2.
Motif Occurrences
Number of Occurrences
The probability for to occur at position will be given in Section 3.1.
3. Mean and Variance for the Count
This section will provide analytical formulae for the mean and the variance of the number of occurrences of a coloured motif in a random graph. It involves the computation of some probabilities of connectedness. The generalisation to the number of occurrences of a set a coloured motifs will be done in the supplementary material.
3.1. Mean Number of Occurrences
where is the occurrence probability of the motif and is given below by (6).
Occurrence Probability
where denotes the probability for a random graph (ErdösRényi model) with vertices and edge probability to be connected (by definition, ).
Connectivity Probability
3.2. Variance of the Number of Occurrences
The terms and are now separately calculated.
where is the multiplicity of in . For instance, if , and then the multiplicity of in equals 2 whereas the multiplicity of equals 1.
Computation of
For the other cases, no general formulae have been found so far but for small values of one can automatically enumerate all the solutions thanks to the edge binary tree, as described below. As an illustration, the case (and ) will be detailed.
where is any subgraph of vertices. Since is typically small, both probabilities can be computed by enumerating all possible subgraphs and . This can be done by traversing the complete edge binary tree associated to the potential edges of that is, to the binary tree whose branches are labelled according to the presence or absence of edges in the subgraph . This tree is composed of levels, one for each potential edge and each internal vertex in this tree has two sons: the left one corresponds to the presence of the corresponding edge in the graph whereas the right one corresponds to its absence. It follows that each path from the root to a leaf corresponds to one of the possible graphs of size . Figure 3 gives an example for . Vertices are labelled , the higher level corresponds to the edge , the middle one corresponds to the edge and the lower level corresponds to the edge . Leaves corresponding to connected graphs are drawn with a square. In practice, the connectedness of a graph can be checked thanks to its adjacency matrix to the power . Indeed, a graph of size with adjacency matrix is connected if and only if contains no zero (every vertex can be reached from any vertex in at most steps). Additionally, the binary tree is built such that all pairs of common vertices between and are at the top levels. The probability of each connected graph of size can then be easily calculated when traversing the tree and likewise for both probabilities appearing in (16).
Finally, we obtained analytical formulae for the variance.
4. Towards the Motif Count Distribution: A Simulated Approach
Aim
Quality of approximation of the count distribution for and . The empirical mean , variance , and cumulative distribution function have been obtained thanks to 10 000 random graphs. are the parameters of the PólyaAeppli distribution. and are the KolmogorovSmirnov distances. For then , is the quantile of the normal distribution (idem for the PólyaAeppli distribution).

 

Motif 





 (%)  (%) 
 (%) 
 (%) 
 (%) 
 (%) 
111  1023.65  27462.66  1021.97  27446.53  0.93  73.37  2.40  0.78  1407.4  1.6  1436  1.1  1533.9  0.23  1591  0.12 
122  767.74  14941.43  766.05  14660.79  0.90  76.08  2.14  0.65  1047.7  1.5  1068  1.0  1140.2  0.25  1181  0.07 
123  614.19  8546.68  615.26  8493.22  0.86  83.12  1.75  0.68  829.6  1.4  845  0.8  900.0  0.18  929  0.08 
114  307.09  5729.89  307.77  5807.09  0.90  30.98  3.20  0.71  485.0  1.5  505  0.8  543.3  0.28  583  0.08 
134  122.84  1305.02  123.06  1311.64  0.83  21.11  3.43  0.78  207.3  1.8  219  0.9  235.0  0.37  257  0.12 
115  61.41  1180.68  61.72  1147.95  0.90  6.30  5.72  0.98  140.5  2.3  160  0.8  166.4  0.57  205  0.06 
244  15.35  85.99  15.29  85.57  0.70  4.63  8.73  1.07  36.8  2.4  43  0.8  43.9  0.81  55  0.12 
245  6.14  27.76  6.20  28.45  0.64  2.22  12.72  1.27  18.6  2.5  23  0.8  22.7  1.09  32  0.10 
345  2.46  6.63  2.51  6.58  0.45  1.39  17.97  0.53  8.5  1.9  11  0.5  10.4  0.77  15  0.09 
155  1.23  6.94  1.22  6.74  0.69  0.37  34.23  5.75  7.2  3.3  12  0.6  9.2  1.56  20  0.05 
444  1.02  2.46  1.02  2.51  0.42  0.59  27.39  3.80  4.7  2.4  7  0.5  5.9  1.48  10  0.09 
355  0.25  0.50  0.25  0.50  0.34  0.16  48.47  0.43  1.9  2.5  3  0.4  2.4  0.96  6  2e05 
455  0.12  0.20  0.13  0.20  0.23  0.09  51.63  0.16  1.2  0.6  2  0.1  1.5  0.65  4  0.03 
555  0.008  0.01  0.007  0.008  0.035  0.007  52.61  2e03  0.2  0.03  0  0.03  0.3  0.03  1  2e05 
Simulation Design
We have simulated 10 000 ErdösRényi random graphs with vertices ( ) and edge probability . Vertices have been randomly coloured with 5 colours ( ) and according to the following colour frequencies: . These choices for , , and allow to get coloured motifs of size 3 with a wide range of expected counts. We have then selected 14 motifs of size 3 to cover both this variety of counts and different multiplicity pattern: , , , , , , , , , , , , and .
Quality of Approximation
To measure this quality, we adopted two criteria: (1) the KolmogorovSmirnov distance which measures the maximal difference between the empirical cumulative distribution function (cdf) and the cdf of the normal or the PólyaAeppli distribution. The closer to 0 the KS distance, the better the approximation. (2) 1 minus the empirical cdf calculated at the and quantiles of the normal or of the PólyaAeppli distribution. The closer to 1% and 0.1% these values, the better the approximation.
Results
Results for different values of and are very similar. We only present here the ones corresponding to and because these values are very close to those observed in real cases such as the metabolic network of E. coli as considered in Lacroix et al. [12]. Nevertheless, all results are presented in the supplementary material.
We can first notice just by eye (see Figure 4) that the normal distribution seems satisfactory for frequent motifs but the rarer the motif, the worse the goodnessoffit. The PólyaAeppli distribution seems to fit quite correctly the count distribution whatever the motif. These initial impressions are emphasised when we look at the KolmogorovSmirnov distances (see Table 1). The ones for the PólyaAeppli distribution are always smaller than those for the normal distribution and sometimes much smaller. In fact, the distance to the normal distribution is quite large for very rare motifs (typically when ). If we now concentrate on the distribution tails by looking at the empirical probabilities to exceed the 99% or 99.9% quantiles and , we can also notice that they are closer to 1% or 0.1% for the PólyaAeppli distribution than for the normal distribution. For extremely rare motifs, quantiles for both 99% and 99.9% could not be correctly calculated because the corresponding PólyaAeppli distribution is both discrete and concentrated around 0. The values for the empirical tails provided in the table are therefore not meaningful in such cases, but thanks to the very small KS distances, we can check that the approximation is still good. Finally, observe that most of the time the normal distribution underestimates the quantile (the empirical right tail is overestimated) leading to false positives.
5. Discussion and Conclusion
In this paper, we proposed a new way to assess the exceptionality of coloured motifs in networks which do not require to perform simulations. Indeed, we were able to establish analytical formulae for the mean and the variance of the count of a coloured motif in an ErdösRényi random graph model. Furthermore, using simulations, we showed that the motif count distribution can be quite accurately approximated with a PólyaAeppli distribution, and that neither the Gaussian nor the Poisson distributions are relevant. Altogether, these results now allow to derive a value for a coloured motif without performing simulations. Clearly, when several motifs have to be tested, which is the case in the context of motif discovery, one has to control for multiple testing. A conservative strategy that is classically used and that we would recommend is then to apply a Bonferroni correction.
In this work, we did not investigate the case of long motifs, but we can anticipate that motifs containing submotifs which are exceptional will tend to be exceptional themselves. This type of phenomenon is also observed for patterns in sequences and a classical way to deal with it is to control for the number of sequence patterns of size (by using a Markov model of order ), when assessing the exceptionality of patterns of size . However, in the case of networks, the problem is far from trivial and it is unclear, even for small values of if the space of random graphs verifying these constraints will not be too small. In the worst case, this space may even be reduced to the observed graph itself.
Also in the case of very rare motifs, the expected distribution of the count is essentially concentrated around 0. Therefore, a single occurrence of such a motif will often be sufficient for it to be considered as exceptional. If we now consider the extreme case of a coloured graph, where each vertex is assigned a different colour, then all possible motifs will be very rare and, therefore, they may all be detected as exceptional. In practical cases, such as for the network representing the metabolic network of the bacterium E. coli, the situation is less dramatic but indeed many colours are present only once. This issue may be partially addressed by considering a random graph model, where the colours and the topology are not independent anymore. This would allow to discriminate between infrequent poorly connected colours and infrequent highly connected colours. Motifs containing the latter type of colours would be expected to have more occurrences and should therefore not be systematically considered as exceptional when they have a single occurrence.
More generally, we considered in this paper a very simple random graph model. Even though we think this work was necessary to establish a framework for accessing the exceptionality of coloured motifs, an important step is now to extend these results to other models of random graphs which better represent the structure of real networks. Different types of models have been proposed in the literature for this purpose, for instance, smallworld networks, scalefree networks, preferential attachment models, and fixed degree distribution models. However, these models do not provide the probabilistic distribution on edges which is required to compute the occurrence probability of a motif and the probability of two nondisjoint occurrences. Moreover, it has been shown that subnetworks of scalefree networks lose the scalefree property [19]. This is a real drawback for modelling biological networks because they usually correspond to the partial knowledge we have of a system and are therefore far from complete. An interesting issue would be to generalise our work to a mixture of ER random graph models. These models seem indeed very flexible and are able to fit nicely biological networks [17].
Finally, we think there is still room for improvement on the approximation of the motif count distribution. Indeed, no theoretical evidence has been found so far supporting the use of a geometric distribution for the clump size. Analytically, getting the third moment and eventually the fourth moment of the count could certainly allow to investigate other distributions.
Declarations
Acknowledgments
The authors would like to thank Etienne Birmelé, JeanJacques Daudin, Catherine Matias, and Stéphane Robin for helpful discussions about the moment calculations. They particularly thank JeanJacques Daudin for providing a MATLAB program to automatically compute the term . They also thank the anonymous reviewers for their helpful comments and suggestions for improving the manuscript. This work has been supported by the ANR (NEMO Project BLAN081_318829, REGLIS Project NT053_45205, and MIRI Project BLAN081_335497) and the ANRBBSRC (MetNet4SysBio Project ANR07BSYS 003 02).
Authors’ Affiliations
References
 Alm E, Arkin AP: Biological networks. Current Opinion in Structural Biology 2003, 13(2):193202. 10.1016/S0959440X(03)000319View ArticleGoogle Scholar
 Jeong H, Tombor B, Albert R, Oltvai ZN, Barabási AL: The largescale organization of metabolic networks. Nature 2000, 407(6804):651654. 10.1038/35036627View ArticleGoogle Scholar
 Maslov S, Sneppen K: Specificity and stability in topology of protein networks. Science 2002, 296(5569):910913. 10.1126/science.1065103View ArticleGoogle Scholar
 Wagner A, Fell DA: The small world inside large metabolic networks. Proceedings of the Royal Society B 2001, 268(1478):18031810. 10.1098/rspb.2001.1711View ArticleGoogle Scholar
 Milo R, ShenOrr SS, Itzkovitz S, Kashtan N, Chklovskii D, Alon U: Network motifs: simple building blocks of complex networks. Science 2002, 298(5594):824827. 10.1126/science.298.5594.824View ArticleGoogle Scholar
 ShenOrr SS, Milo R, Mangan S, Alon U: Network motifs in the transcriptional regulation network of Escherichia coli . Nature Genetics 2002, 31(1):6468. 10.1038/ng881View ArticleGoogle Scholar
 Janson S, Łuczak T, Ruciński A: Random Graphs. WileyInterscience, New York, NY, USA; 2000.View ArticleMATHGoogle Scholar
 Stark D: Compound Poisson approximations of subgraph counts in random graphs. Random Structures & Algorithms 2001, 18(1):3960. 10.1002/10982418(200101)18:1<39::AIDRSA4>3.0.CO;2BView ArticleMathSciNetMATHGoogle Scholar
 Itzkovitz S, Milo R, Kashtan N, Ziv G, Alon U: Subgraphs in random networks. Physical Review E 2003, 68(2):8.Google Scholar
 Camacho J, Stouffer DB, Amaral LAN: Quantitative analysis of the local structure of food webs. Journal of Theoretical Biology 2007, 246(2):260268. 10.1016/j.jtbi.2006.12.036View ArticleMathSciNetGoogle Scholar
 Picard F, Daudin JJ, Koskas M, Schbath S, Robin S: Assessing the exceptionality of network motifs. Journal of Computational Biology 2008, 15(1):120. 10.1089/cmb.2007.0137View ArticleMathSciNetGoogle Scholar
 Lacroix V, Fernandes CG, Sagot MF: Motif search in graphs: application to metabolic networks. IEEE/ACM Transactions on Computational Biology and Bioinformatics 2006, 3(4):360368. 10.1109/TCBB.2006.55View ArticleGoogle Scholar
 Fellows MR, Fertin G, Hermelin D, Vialette S: Sharp tractability borderlines for finding connected motifs in vertexcolored graphs. Proceedings of the 34th International Colloquium on Automata, Languages and Programming (ICALP '07), July 2007, Wroclaw, Poland, Lecture Notes in Computer Science 4596: 340351.MathSciNetGoogle Scholar
 Lacroix V, Cottret L, Rogier O, Fernandes C, Jourdan F, Sagot MF: Motus: a software and a webserver for the search and enumeration of nodelabelled connected subgraphs in biological networks. submittedGoogle Scholar
 Johnson NL, Kotz S, Kemp AW: Univariate Discrete Distributions. John Wiley & Sons, New York, NY, USA; 1992.MATHGoogle Scholar
 Schbath S: Compound Poisson approximation of word counts in DNA sequences. ESAIM: Probability and Statistics 1995, 1: 116. 10.1051/ps:1997100View ArticleMathSciNetMATHGoogle Scholar
 Daudin JJ, Picard F, Robin S: A mixture model for random graphs. Statistics and Computing 2008, 18(2):173183. 10.1007/s1122200790467View ArticleMathSciNetGoogle Scholar
 Gilbert EN: Random graphs. The Annals of Mathematical Statistics 1959, 30(4):11411144. 10.1214/aoms/1177706098View ArticleMATHGoogle Scholar
 Stumpf MPH, Wiuf C, May RM: Subnets of scalefree networks are not scalefree: sampling properties of networks. Proceedings of the National Academy of Sciences of the United States of America 2005, 102(12):42214224. 10.1073/pnas.0501179102View ArticleGoogle Scholar
Copyright
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.