Table 3 FCS-immunoassay versus ELISA
Parameter | FCS-assay | ELISA |
|---|---|---|
Sensitivity | μM to sub-nM | nM to sub-nM |
Sample volume required | Approximately 20 μl | 100 μl |
Measurement time | Approximately 25 min | >2.5 h |
Immunocomplex formation | In solution | On the surface |
Washing steps required | No | Yes |
Calibration curve required | Yes (confocal volume) | Yes |
Detection mode | Size-based fluctuations in signal intensities | Changes in signal intensity |