Table 1 Specificity of siRNA target prediction
shRNA (passenger) | Target | Reference | Target predicted by the SPICEa |
|---|---|---|---|
GAUUAUCCAAAGAGGUUCU | RPS6KA6 | Berns et al. [2] | RPS6KA6 (1, 0) |
UGGUUGAUGAGCCAAUGGA | RPS6KA6 | Berns et al. [2] | RPS6KA6 (1, 0) |
GUACGGCCGUAGUCUCAAG | HTATIP | Berns et al. [2] | KAT5 (1, 0): identical to HTATIP |
CAAACGUCUGGAUGAAUGG | HTATIP | Berns et al. [2] | KAT5 (1, 0): identical to HTATIP |
GCCAUCCAGAUGGACUUUC | HDAC4 | Berns et al. [2] | HDAC4 (1, 0) |
GCAUGUGUUUCUGCCUUGC | HDAC4 | Berns et al. [2] | HDAC4 (1, 0) |
GCAGCAGAGGUGAUUCUGC | CCT2 | Berns et al. [2] | CCT2 (1, 0) |
GAGAGGCGCUGUUGAGUUC | CCT2 | Berns et al. [2] | CCT2 (1, 0) |
GAGUACAUUCUGCCUUGCU | KIAA0828 | Berns et al. [2] | AHCYL2 (1, 0): identical to KIAA0828 |
GCUACAUCAAGAACUCAGC | KIAA0828 | Berns et al. [2] | AHCYL2 (1, 0): identical to KIAA0828 |
GAGACCTACCTCCGGATCA | PLK1 | Jackson et al. [16] | PLK1 (1, 0) |