Two examples of univariate StepMiner. (a) Scatter plot of ESR1’s gene expression and methylation (cg20253551). The dotted lines indicate thresholds identified by StepMiner. (b) Samples are ordered according to ESR1 expression, and a step function is fitted to the ordered expression data and identify a threshold to dichotomize expression. (c) A step function is fitted to the ordered methylation data and identify a threshold to binarize methylation.