In vitro detection of adrenocorticotropic hormone levels by fluorescence correlation spectroscopy immunoassay for mathematical modeling of glucocorticoid-mediated feedback mechanisms

EURASIP Journal on Bioinformatics and Systems Biology

Table 1 In vitro studies in AtT-20s within 22 h

CRH (nM)	Cort (nM)	Cell population (cells/ml)	ACTH (nM)
0	0	205164 ± 12889	15.016 ± 0.201
5	0	335938 ± 20522	16.764 ± 0.593
10	0	324219 ± 23935	17.427 ± 0.422
36	0	302084 ± 28168	16.146 ± 0.564
54	0	237500 ± 15630	15.364 ± 0.575
10	50	312500 ± 28125	16.673 ± 0.467
10	100	298438 ± 20625	15.679 ± 0.337

Cell proliferation and ACTH response to various doses of extracellular CRH and cortisol within/over 22 h of incubation (114 h total incubation time). Optimal concentrations for CRH stimulation and cortisol inhibition of ACTH secretion were used for studying feedback regulation of ACTH release (bold values). Deviations calculated by the t-test distribution, α = 0.1.