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EURASIP Journal on Bioinformatics and Systems Biology
Figure 4 | EURASIP Journal on Bioinformatics and Systems Biology

Figure 4

From: In vitro detection of adrenocorticotropic hormone levels by fluorescence correlation spectroscopy immunoassay for mathematical modeling of glucocorticoid-mediated feedback mechanisms

Figure 4

Fast and delayed negative feedback regulation of ACTH release in vitro . A typical HPA response to a strong extracellular stress signal (10-nM CRH) is simulated in AtT-20 pituitaries in vitro by adding 100-nM cortisol. Already minutes after cortisol incubation an inhibition and a delay of extracellular ACTH secretion is detectable (blue circular data points), which is supposed to be caused by a fast negative feedback mechanism. Long-term inhibitory effects of cortisol to ACTH secretion (interrupted line) were not analyzed. FCS datasets (a) are consistent with ELISA control measurements (b). Deviations calculated by the t-test distribution, α = 0.1.

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