The relationships between kinetic constants and frequencies of two AA. The multiple linear regressions showing changes of the log-values of kinetic constants as dependent variables upon the frequencies of occurrence for two AA in the yeast S. cerevisiae sequences, where k cat is the catalytic constant (A), K M is the Michaelis-Menten constant (C), and k sp = k cat/K M is the specificity constant (E). The observed versus predicted plots (B,D,F) for the values of dependent variables (k cat, K M, and k sp, respectively). The predicted values were calculated from the regression equations: log(k cat) = 5.556 –1.620*M −0.984*W (R adj.2 = 82.88%, p = 0.0000); log(K M) = 8.593 –0.596*N −0.998*D (R adj.2 = 53.72%, p = 0.0039); log(k cat/K M) = 0.818 +0.501*A −1.736*H (R adj.2 = 46.50%, p = 0.0068). All the multiple and pair correlations (A–F) are significant at the non-parametric assessment (Kendall's τ, Spearman's ρ correlation coefficients).