Table 1 Mechanistic model of the Ras/cAMP/PKA pathway in S. cerevisiae
No. | Reagents | Products | Constant c i |
|---|---|---|---|
r 1 | Ras2-GDP + Cdc25 | Ras2-GDP-Cdc25 | 1.0 |
r 2 | Ras2-GDP-Cdc25 | Ras2-GDP + Cdc25 | 1.0 |
r 3 | Ras2-GDP-Cdc25 | Ras2-Cdc25 + GDP | 1.5 |
r 4 | Ras2-Cdc25 + GDP | Ras2-GDP-Cdc25 | 1.0 |
r 5 | Ras2-Cdc25 + GTP | Ras2-GTP-Cdc25 | 1.0 |
r 6 | Ras2-GTP-Cdc25 | Ras2-Cdc25 + GTP | 1.0 |
r 7 | Ras2-GTP-Cdc25 | Ras2-GTP + Cdc25 | 1.0 |
r 8 | Ras2-GTP + Cdc25 | Ras2-GTP-Cdc25 | 1.0 |
r 9 | Ras2-GTP + Ira2 | Ras2-GTP-Ira2 | ∗1.0 ×10−2 |
r 10 | Ras2-GTP-Ira2 | Ras2-GDP + Ira2 | ∗2.5 ×10−1 |
r 11 | Ras2-GTP + Cyr1 | Ras2-GTP-Cyr1 | 1.0 ×10−3 |
r 12 | Ras2-GTP-Cyr1 + ATP | Ras2-GTP-Cyr1 + cAMP | 2.1 ×10−6 |
r 13 | Ras2-GTP-Cyr1 + Ira2 | Ras2-GDP + Cyr1 + Ira2 | 1.0 ×10−3 |
r 14 | cAMP + PKA | cAMP-PKA | 1.0 ×10−5 |
r 15 | cAMP + cAMP-PKA | (2cAMP)-PKA | 1.0 ×10−5 |
r 16 | cAMP + (2cAMP)-PKA | (3cAMP)-PKA | 1.0 ×10−5 |
r 17 | cAMP + (3cAMP)-PKA | (4cAMP)-PKA | 1.0 ×10−5 |
r 18 | (4cAMP)-PKA | cAMP + (3cAMP)-PKA | 1.0 ×10−1 |
r 19 | (3cAMP)-PKA | cAMP + (2cAMP)-PKA | 1.0 ×10−1 |
r 20 | (2cAMP)-PKA | cAMP + cAMP-PKA | 1.0 ×10−1 |
r 21 | cAMP-PKA | cAMP + PKA | 1.0 ×10−1 |
r 22 | (4cAMP)-PKA | C + C + R-2cAMP + R-2cAMP | 1.0 |
r 23 | R-2cAMP | R + cAMP + cAMP | 1.0 |
r 24 | R + C | R-C | 7.5 ×10−1 |
r 25 | R-C + R-C | PKA | 1.0 |
r 26 | C + Pde1 | C + Pde1p | 1.0 ×10−6 |
r 27 | cAMP + Pde1p | cAMP-Pde1p | 1.0 ×10−1 |
r 28 | cAMP-Pde1p | cAMP + Pde1p | 1.0 ×10−1 |
r 29 | cAMP-Pde1p | AMP + Pde1p | 7.5 |
r 30 | Pde1p + PPA2 | Pde1 + PPA2 | 1.0 ×10−4 |
r 31 | cAMP + Pde2 | cAMP-Pde2 | 1.0 ×10−4 |
r 32 | cAMP-Pde2 | cAMP + Pde2 | 1.0 |
r 33 | cAMP-Pde2 | AMP + Pde2 | 1.7 |
r 34 | C + Cdc25 | C + Cdc25p | 1.0 |
r 35 | Cdc25p + PPA2 | Cdc25 + PPA2 | 1.0 ×10−2 |
r 36 | Ira2 + C | Ira2p + C | 1.0 ×10−3 |
r 37 | Ras2-GTP + Ira2p | Ras2-GTP-Ira2p | 1.25 |
r 38 | Ras2-GTP-Ira2p | Ras2-GDP + Ira2p | 2.5 |
r 39 | Ira2p | Ira2 | 10.0 |