Choice of weight matrix reference set. (a, b) The test set of 3470 annAUGs with 5'UTR ≥ 200 is displayed using two different reference sets to construct weight matrices: (i) (blue) and (ii) all cDNAs with 5'UTRs 100 to 199 (red). (c, d) Equivalent analysis using a test set of 1922 annAUGs (5'UTRs 100 to 199) and the reference sets (i) (blue) and (ii) all cDNAs with 5′UTR ≥ 200 (red). In both analyses, using the 0-upAUG reference set expands the range of relative individual information scores. (a, c) TRII score distributions. (b, d) corresponding cumulative distributions.