Figure 2

Relative individual information score distributions (a) and corresponding cumulative distributions (b). The annAUGs of the full set of cDNAs with 5^'UTR ≥ 200 were used as a reference set to construct the weight matrix for nucleotide positions −20 to 20. Three test sets were compared: (i) 0upAUGs, 5^'UTR ≥ 200 (red); (ii) 687 cDNAs with at least 10 upAUGs, 5^'UTR ≥ 200 (blue); (iii) AUGs surrounded with random sequences conforming to the 5^'UTR background frequencies (grey). In this example, the reference set includes the 0-upAUG test set (red); however, the use of nonoverlapping reference and test sets is preferred (see text).