Table 1 12 parameters used in [18] for predicting hybridization affinities between DNA sequence pairs.

Probe sequence length, Target sequence length

Probe GC content, target GC content

Smith-Waterman score: computed from the scoring system used in the SW alignment

-value: probability that the SW score occurred by chance

Percent identity: percentage of matched bases in the aligned region after SW alignment

Length of the SW alignment

Gibbs free energy for probe DNA folding

Hamming distance between probe and target

Length of longest contiguous matched segment in a SW alignment

GC content in the longest contiguous segment