Table 2 Validation of FCS results
Time (min) | ACTH (FCS) | ACTH (ELISA) | ||
|---|---|---|---|---|
% changea | % dev | % change to FCS | % dev | |
Basal | ||||
0 | 0.00 | ±1.28 | −3.39 | ±2.91 |
1320 | +7.48 | ± 1.29 | −1.86 | ±2.16 |
+10 nM CRH | ||||
1 | +4.79 | ±1.82 | −0.91 | ±3.11 |
5 | +8.08 | ±1.39 | −1.16 | ±3.35 |
15 | +8.62 | ±1.36 | +2.77 | ±3.29 |
30 | +9.51 | ±1.43 | +4.05 | ±2.96 |
60 | +11.18 | ±1.62 | +4.61 | ±2.12 |
120 | +15.50 | ±1.86 | +2.97 | ±2.27 |
1320 | +24.74 | ±2.32 | +2.63 | ±2.31 |
+10 nM CRH + 100 nM cortisol | ||||
1 | +1.16 | ±2.03 | −3.77 | ±3.58 |
5 | +2.08 | ±2.88 | −3.68 | ±4.12 |
15 | +3.02 | ±2.85 | −2.97 | ±3.38 |
30 | +4.94 | ±1.75 | −1.80 | ±2.44 |
60 | +6.80 | ±1.63 | −0.81 | ±3.01 |
120 | +9.40 | ±2.31 | +1.52 | ±2.33 |
1320 | +12.23 | ±2.07 | +3.10 | ±2.41 |